Immunoblot
1. 100 ug Human lung microsome lysatePrimary Antibody Dilution: 1: 1000Secondary Antibody: Secondary Antibody Dilution: 1: 10000Gene Name: COMTSubmitted by: Jing Peng, Fox Chase Cancer CenteWestern blot
COMT antibody - middle region validated by WB using rat liver, mouse lung, human brain at 1:1000.Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Stomach| Product Name | Rabbit anti-COMT polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-COMT antibody: synthetic peptide directed towards the middle region of human COMT |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COMT |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | Catechol-O-methyltransferase |
| Gene Summary | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathwa |
| Molecular Weight(MW) | 30kDa |
| Sequence | 271 amino acids |
Immunoblot
1. 100 ug Human lung microsome lysatePrimary Antibody Dilution: 1: 1000Secondary Antibody: Secondary Antibody Dilution: 1: 10000Gene Name: COMTSubmitted by: Jing Peng, Fox Chase Cancer Cente
Western blot
COMT antibody - middle region validated by WB using rat liver, mouse lung, human brain at 1:1000.
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Stomach| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
