Immunohistochemical
ImmunohistochemicalWestern blot
2.5 ug/mlPositive Control: JurkatImmunoblot
Lane 1: 50ug HeLa lysateLane 2: 50ug 293T lysateLane 3: 50ug K562 lysateLane 4: 50ug MDA-MB-231 lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: SOD1Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences| Product Name | Rabbit anti-SOD1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALS, ALS1, IPOA, SOD, homodimer, hSod1 |
| Immunogen | The immunogen for anti-SOD1 antibody: synthetic peptide directed towards the N terminal of human SOD1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SOD1 |
|---|---|
| Gene Synonyms | ALS ALS1 IPOA SOD homodimer hSod1 |
| Gene Full Name | Superoxide dismutase 1, soluble |
| Gene Summary | SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in its gene have been implicated as causes of familial amyotrophic lateral sclerosis.The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. |
| Alternative Names | ALS ALS1 IPOA SOD homodimer hSod1 |
| Molecular Weight(MW) | 16kDa |
| Sequence | 154 amino acids |
Immunohistochemical
Immunohistochemical
Western blot
2.5 ug/mlPositive Control: Jurkat
Immunoblot
Lane 1: 50ug HeLa lysateLane 2: 50ug 293T lysateLane 3: 50ug K562 lysateLane 4: 50ug MDA-MB-231 lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: SOD1Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
