Immunohistochemistry
Anti-PSME3 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.Western blot
Host: Rabbit Target Name: HIRIP3 Sample Tissue: 293T Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: Hela Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-PSME3 Antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ki, PA28-gamma, PA28G, REG-GAMMA |
| Immunogen | The immunogen for Anti-PSME3 antibody is: synthetic peptide directed towards the N-terminal region of Human PSME3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSME3 |
|---|---|
| Gene Synonyms | Ki PA28-gamma PA28G REG-GAMMA |
| Gene Full Name | Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) |
| Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME3 is the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. |
| Alternative Names | Ki PA28-gamma PA28G REG-GAMMA |
| Molecular Weight(MW) | 31kDa |
| Sequence | 267 amino acids |
Immunohistochemistry
Anti-PSME3 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot
Host: Rabbit Target Name: HIRIP3 Sample Tissue: 293T Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: Hela Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
