Western blot
Data Provided by Dr. Margarida Duarte, IBMC PortugalThe antibody in Neurospora crassa mitochondria and post-mitochondrial supernatants.In this filamentous fungus a single gene encodes cytosolic and mitochondrial valyl tRNA synthetase.We used a 1:1000 dilution of the primary antibody (overnight incubation at 4ºC) followed by an HRP conjugated secondary antibody. Chemiluminescence detection was performed using the 'western lightning Ultra'from PerkinElmer.Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-VARS polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G7A, VARS2, VARS1 |
| Immunogen | The immunogen for anti-VARS antibody: synthetic peptide directed towards the middle region of human VARS |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VARS |
|---|---|
| Gene Synonyms | G7A VARS2 VARS1 |
| Gene Full Name | Valyl-tRNA synthetase |
| Gene Summary | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. VARS belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | G7A VARS2 VARS1 |
| Molecular Weight(MW) | 140kDa |
| Sequence | 1264 amino acids |
Western blot
Data Provided by Dr. Margarida Duarte, IBMC PortugalThe antibody in Neurospora crassa mitochondria and post-mitochondrial supernatants.In this filamentous fungus a single gene encodes cytosolic and mitochondrial valyl tRNA synthetase.We used a 1:1000 dilution of the primary antibody (overnight incubation at 4ºC) followed by an HRP conjugated secondary antibody. Chemiluminescence detection was performed using the 'western lightning Ultra'from PerkinElmer.
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
