Western blot
Host: Rabbit Target Name: Melk Sample Tissue: Mouse Testis lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-Melk Antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI327312, MPK38, mKIAA0175 |
| Immunogen | The immunogen for Anti-Melk antibody is: synthetic peptide directed towards the N-terminal region of Mouse Melk |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Melk |
|---|---|
| Gene Synonyms | AI327312 MPK38 mKIAA0175 |
| Gene Full Name | maternal embryonic leucine zipper kinase |
| Gene Summary | Melk is a Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Melk Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It Plays a key role in cell proliferation. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. It's also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus and may also play a role in primitive hematopoiesis. |
| Alternative Names | AI327312 MPK38 mKIAA0175 |
| Molecular Weight(MW) | 70kDa |
| Sequence | 643 amino acids |
Western blot
Host: Rabbit Target Name: Melk Sample Tissue: Mouse Testis lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
