Immunohistochemistry
Researcher: Dr. Crissy Dudgeon, Ph.D., CINJApplication: IHCSpecies+tissue/cell type: Human lung adenocarcinoma cell line A549Primary antibody dilution: 1:100Secondary antibody: Goat anti-rabbit AlexaFluor 488Secondary antibody dilution: 1:400Immunoblot
1. 30 ug MiaPaca-2 cell lysate2. 30 ug Panc-1 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:4000Gene Name: LDHBSubmitted by: Dr. Crissy Dudgeon, Ph.D., CINJ| Product Name | Rabbit anti-LDHB Antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LDH-H, TRG-5, LDH-B, LDHBD |
| Immunogen | The immunogen for Anti-LDHB antibody is: synthetic peptide directed towards the C-terminal region of Human LDHB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LDHB |
|---|---|
| Gene Synonyms | LDH-H TRG-5 LDH-B LDHBD |
| Gene Full Name | Lactate dehydrogenase B |
| Gene Summary | LDHB belongs to the LDH/MDH superfamily, LDH family. Defects in LDHB are a cause of hereditary LDHB deficiency. LDHB may also have roles in progression of medulloblastoma. |
| Alternative Names | LDH-H TRG-5 LDH-B LDHBD |
| Molecular Weight(MW) | 37kDa |
| Sequence | 334 amino acids |
Immunohistochemistry
Researcher: Dr. Crissy Dudgeon, Ph.D., CINJApplication: IHCSpecies+tissue/cell type: Human lung adenocarcinoma cell line A549Primary antibody dilution: 1:100Secondary antibody: Goat anti-rabbit AlexaFluor 488Secondary antibody dilution: 1:400
Immunoblot
1. 30 ug MiaPaca-2 cell lysate2. 30 ug Panc-1 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:4000Gene Name: LDHBSubmitted by: Dr. Crissy Dudgeon, Ph.D., CINJ| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
