Immunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :VIM: Red DAPI:BlueGene Name :VIMSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunofluorescence
submitted by:Carl LundinStanford UniversityThe Vimentin antibody ParaffinP050 works well for visualization of the vimentin cytoskeleton (see attached images). We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.Western blot
Host: Rabbit Target Name: VIM Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/mlWestern blot
0.2-1 ug/mlPositive Control: Human brain| Product Name | Rabbit anti-VIM polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ36605 |
| Immunogen | The immunogen for anti-VIM antibody: synthetic peptide directed towards the N terminal of human VIM |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VIM |
|---|---|
| Gene Synonyms | FLJ36605 |
| Gene Full Name | Vimentin |
| Gene Summary | Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | FLJ36605 |
| Molecular Weight(MW) | 54kDa |
| Sequence | 466 amino acids |
Immunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :VIM: Red DAPI:BlueGene Name :VIMSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunofluorescence
submitted by:Carl LundinStanford UniversityThe Vimentin antibody ParaffinP050 works well for visualization of the vimentin cytoskeleton (see attached images). We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.
Western blot
Host: Rabbit Target Name: VIM Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/ml
Western blot
0.2-1 ug/mlPositive Control: Human brain| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
