Western blot
Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution :Western blot
0.2-1 ug/ml| Product Name | DNMT3B antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-DNMT3B antibody: synthetic peptide directed towards the middle region of human DNMT3B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Species Reactivity | Bovine,Dog,Guinea Pig,Horse,Human,Mouse,Pig,Rabbit,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNMT3B |
|---|---|
| Gene Synonyms | ICF M.HsaIIIB ICF1 |
| Gene Full Name | DNA (cytosine-5-)-methyltransferase 3 beta |
| Gene Summary | DNMT3B is required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. DNMT3B may preferentially methylate nucleosomal DNA within the nucleosome core region. DNMT3B may function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. DNMT3B seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, DNMT3B is involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. |
| Alternative Names | ICF M.HsaIIIB ICF1 |
| Molecular Weight(MW) | 86kDa |
| Sequence | 770 amino acids |
Western blot
Lane 1: 20ug mouse mesenchymal stem cell lysate Primary Antibody Dilution :
Western blot
0.2-1 ug/ml| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
