Immunoblot
1. 30 ug control HEK-293T lysate2. 30 ug hPCL3L-FLAG transfected HEK-293T lysate3. 30 ug hPCL3L-MYC transfected HEK-293T lysate4. 30 ug hPCL3L-HA transfected HEK-293T lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: PHF19Submitted by: AnonymousImmunoblot
1. 30 ug HBEC lysate2. 30 ug A549 lysate3. 30 ug Calu-6 lysate4. 30 ug NCI358 lysate5. 30 ug NCI841 lysate6. 30 ug NCI1299 lysate7. 30 ug NCI1650 lysate8. 30 ug NCI1975 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: PHF19Submitted by: AnonymousWestern blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Product Name | Rabbit anti-PHF19 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC131698, MGC149712, MGC149713, MGC23929, PCL3, MTF2L1 |
| Immunogen | The immunogen for anti-PHF19 antibody: synthetic peptide directed towards the C terminal of human PHF19 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHF19 |
|---|---|
| Gene Synonyms | MGC131698 MGC149712 MGC149713 MGC23929 PCL3 MTF2L1 |
| Gene Full Name | PHD finger protein 19 |
| Gene Summary | PHF19 contains 2 PHD-type zinc fingers. It acts as a transcritpional repressor. Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter. |
| Alternative Names | MGC131698 MGC149712 MGC149713 MGC23929 PCL3 MTF2L1 |
| Molecular Weight(MW) | 22kDa |
| Sequence | 207 amino acids |
| Entrez Gene | 26147 |
|---|---|
| SwissProt ID | Q5T6S3-2 |
| Protein Accession | NP_001009936 |
Immunoblot
1. 30 ug control HEK-293T lysate2. 30 ug hPCL3L-FLAG transfected HEK-293T lysate3. 30 ug hPCL3L-MYC transfected HEK-293T lysate4. 30 ug hPCL3L-HA transfected HEK-293T lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: PHF19Submitted by: Anonymous
Immunoblot
1. 30 ug HBEC lysate2. 30 ug A549 lysate3. 30 ug Calu-6 lysate4. 30 ug NCI358 lysate5. 30 ug NCI841 lysate6. 30 ug NCI1299 lysate7. 30 ug NCI1650 lysate8. 30 ug NCI1975 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:3000Gene Name: PHF19Submitted by: Anonymous
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
