Western blot
0.2-1 ug/mlPositive Control: Human Lung| Product Name | Rabbit anti-SHC1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ26504, SHC, SHCA, p52SHC, p66, p66SHC |
| Immunogen | The immunogen for anti-SHC1 antibody: synthetic peptide directed towards the C terminal of human SHC1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SHC1 |
|---|---|
| Gene Synonyms | FLJ26504 SHC SHCA p52SHC p66 p66SHC |
| Gene Full Name | SHC (Src homology 2 domain containing) transforming protein 1 |
| Gene Summary | SHC1 is a signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span. |
| Alternative Names | FLJ26504 SHC SHCA p52SHC p66 p66SHC |
| Molecular Weight(MW) | 52kDa |
| Sequence | 474 amino acids |
Western blot
0.2-1 ug/mlPositive Control: Human Lung| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
