Immunofluorescence
Sample Type: outer mouse plexiform layerRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale UniversityWestern blot
0.2-1 ug/mlPositive Control: Hela cell lysateImmunofluorescence
Sample Type: complete mouse retina sectionsRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale University| Product Name | Rabbit anti-CTBP2 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-CTBP2 antibody: synthetic peptide directed towards the C terminal of human CTBP2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGoatHorseHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTBP2 |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | C-terminal binding protein 2 |
| Gene Summary | This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely.This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. |
| Molecular Weight(MW) | 106kDa |
| Sequence | 985 amino acids |
Immunofluorescence
Sample Type: outer mouse plexiform layerRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale University
Western blot
0.2-1 ug/mlPositive Control: Hela cell lysate
Immunofluorescence
Sample Type: complete mouse retina sectionsRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale University| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
