Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Hela cell lysate| Product Name | Rabbit anti-CDC45L polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC45, CDC45L2, PORC-PI-1, CDC45L |
| Immunogen | The immunogen for anti-CDC45L antibody: synthetic peptide directed towards the C terminal of human CDC45L |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Bovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC45L |
|---|---|
| Gene Synonyms | CDC45 CDC45L2 PORC-PI-1 CDC45L |
| Gene Full Name | Cell division cycle 45 homolog (S. cerevisiae) |
| Gene Summary | CDC45L was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | CDC45 CDC45L2 PORC-PI-1 CDC45L |
| Molecular Weight(MW) | 65kDa |
| Sequence | 566 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
