Immunoblot
Lane 1: Human lung microsome lysateLane 2-5: 150 ug mouse lung microsome lysatePrimary Antibody Dilution: 1: 1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1: 10000Gene Name: CYP1B1Submitted by: Jing Peng, Fox Chase Cancer CenteWestern blot
0.2-1 ug/mlPositive Control: Human Live| Product Name | Rabbit anti-CYP1B1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CP1B, GLC3A, P4501B1, CYPIB1 |
| Immunogen | The immunogen for anti-CYP1B1 antibody: synthetic peptide directed towards the middle region of human CYP1B1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP1B1 |
|---|---|
| Gene Synonyms | CP1B GLC3A P4501B1 CYPIB1 |
| Gene Full Name | Cytochrome P450, family 1, subfamily B, polypeptide 1 |
| Gene Summary | CYP1B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP1B1 localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | CP1B GLC3A P4501B1 CYPIB1 |
| Molecular Weight(MW) | 61kDa |
| Sequence | 543 amino acids |
Immunoblot
Lane 1: Human lung microsome lysateLane 2-5: 150 ug mouse lung microsome lysatePrimary Antibody Dilution: 1: 1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1: 10000Gene Name: CYP1B1Submitted by: Jing Peng, Fox Chase Cancer Cente
Western blot
0.2-1 ug/mlPositive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
