Western blot
Sample Type: HEK 293 (10ug)Primary Dilution: 1:1000Secondary Antibody: conjugated goat anti-rabbitSecondary Dilution: 1:10,000Image Submitted By: Amy GrayBrigham Young UniversityWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate| Product Name | Rabbit anti-CCT7 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCT-ETA, Ccth, MGC110985, Nip7-1, TCP-1-eta, CCTH, CCTETA, NIP7-1, TCP1ETA |
| Immunogen | The immunogen for anti-CCT7 antibody: synthetic peptide directed towards the middle region of human CCT7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCT7 |
|---|---|
| Gene Synonyms | CCT-ETA Ccth MGC110985 Nip7-1 TCP-1-eta CCTH CCTETA NIP7-1 TCP1ETA |
| Gene Full Name | Chaperonin containing TCP1, subunit 7 (eta) |
| Gene Summary | CCT7 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene, but only two of them have been characterized to date. |
| Alternative Names | CCT-ETA Ccth MGC110985 Nip7-1 TCP-1-eta CCTH CCTETA NIP7-1 TCP1ETA |
| Molecular Weight(MW) | 60kDa |
| Sequence | 543 amino acids |
Western blot
Sample Type: HEK 293 (10ug)Primary Dilution: 1:1000Secondary Antibody: conjugated goat anti-rabbitSecondary Dilution: 1:10,000Image Submitted By: Amy GrayBrigham Young University
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
