Immunohistochemistry
Immunohistochemistry with HK2 cell lysate tissueImmunohistochemistry
Immunohistochemistry with Human Placenta lysate tissue at an antibody concentration of 5.0ug/ml using anti-CYP11A1 antibodyWestern blot
Host: Rabbit Target Name: FAM46C Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: Hela Antibody Dilution: 1.0ug/mlWestern blot
CYP11A1 antibody - middle region validated by WB using Hela cell lysate at 1ug/ml.| Product Name | Rabbit anti-CYP11A1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CYP11A, P450SCC, CYPXIA1 |
| Immunogen | The immunogen for anti-CYP11A1 antibody: synthetic peptide directed towards the middle region of human CYP11A1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP11A1 |
|---|---|
| Gene Synonyms | CYP11A P450SCC CYPXIA1 |
| Gene Full Name | Cytochrome P450, family 11, subfamily A, polypeptide 1 |
| Gene Summary | CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP11A1 localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. |
| Alternative Names | CYP11A P450SCC CYPXIA1 |
| Molecular Weight(MW) | 57kDa |
| Sequence | 521 amino acids |
Immunohistochemistry
Immunohistochemistry with HK2 cell lysate tissue
Immunohistochemistry
Immunohistochemistry with Human Placenta lysate tissue at an antibody concentration of 5.0ug/ml using anti-CYP11A1 antibody
Western blot
Host: Rabbit Target Name: FAM46C Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: Hela Antibody Dilution: 1.0ug/ml
Western blot
CYP11A1 antibody - middle region validated by WB using Hela cell lysate at 1ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM114570