Western blot
PRKAA1 antibody - middle region validated by WB using Rat liver and Mouse muscle at 1:1000.Western blot
Sample Type: rat hepatocyte (50ug)Priamry Dilution: 1:4000Secondary Antibody: Donkey anti-Rabbit HRPSecondary Dilution: 1:10,000Image Submitted By: Dustin Singer,INRA/AgroParistechWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: COLO205 cell lysate| Product Name | Rabbit anti-PRKAA1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMPK, AMPKa1, MGC33776, MGC57364 |
| Immunogen | The immunogen for anti-PRKAA1 antibody: synthetic peptide directed towards the middle region of human PRKAA1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseMousePigRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKAA1 |
|---|---|
| Gene Synonyms | AMPK AMPKa1 MGC33776 MGC57364 |
| Gene Full Name | Protein kinase, AMP-activated, alpha 1 catalytic subunit |
| Gene Summary | PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Alternative Names | AMPK AMPKa1 MGC33776 MGC57364 |
| Molecular Weight(MW) | 63kDa |
| Sequence | 559 amino acids |
Western blot
PRKAA1 antibody - middle region validated by WB using Rat liver and Mouse muscle at 1:1000.
Western blot
Sample Type: rat hepatocyte (50ug)Priamry Dilution: 1:4000Secondary Antibody: Donkey anti-Rabbit HRPSecondary Dilution: 1:10,000Image Submitted By: Dustin Singer,INRA/AgroParistech
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: COLO205 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM115405