Immunofluorescence
ImmunofluorescenceWestern blot
Positive Control: Lane 1: 80ug rat brain extract Lane 2: 80ug mouse brain extract Primary Antibody Dilution : 1:500Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR BioscienceSecondry Antibody Dilution : 1:20,000Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Hela cell lysate| Product Name | Rabbit anti-IL1B polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-1, IL1-BETA, IL1F2 |
| Immunogen | The immunogen for anti-IL1B antibody: synthetic peptide directed towards the N terminal of human IL1B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanMouseRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL1B |
|---|---|
| Gene Synonyms | IL-1 IL1-BETA IL1F2 |
| Gene Full Name | Interleukin 1, beta |
| Gene Summary | IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. The gene encoding IL1B and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
| Alternative Names | IL-1 IL1-BETA IL1F2 |
| Molecular Weight(MW) | 17 |
| Sequence | 269 amino acids |
Immunofluorescence
Immunofluorescence
Western blot
Positive Control: Lane 1: 80ug rat brain extract Lane 2: 80ug mouse brain extract Primary Antibody Dilution : 1:500Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR BioscienceSecondry Antibody Dilution : 1:20,000Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM115584