Western blot
Host: Rabbit Target Name: GNAI1 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: GNAI1 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/mlWestern blot
Sample Type: Nthy-ori cell lysate (50ug)Primary Dilution: 1:1000Secondary Antibody: anti-rabbit HRPSecondary Dilution: 1:2000Image Submitted By: AnonymousWestern blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain| Product Name | Rabbit anti-GNAI1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gi |
| Immunogen | The immunogen for anti-GNAI1 antibody: synthetic peptide directed towards the middle region of human GNAI1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GNAI1 |
|---|---|
| Gene Synonyms | Gi |
| Gene Full Name | Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 |
| Gene Summary | Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1); and transducin-1 (GNAT1) and transducin-2 (GNAT2), proteins involved in phototransduction in retinal rods and cones, respectively.Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | Gi |
| Molecular Weight(MW) | 40kDa |
| Sequence | 354 amino acids |
Western blot
Host: Rabbit Target Name: GNAI1 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: GNAI1 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/ml
Western blot
Sample Type: Nthy-ori cell lysate (50ug)Primary Dilution: 1:1000Secondary Antibody: anti-rabbit HRPSecondary Dilution: 1:2000Image Submitted By: Anonymous
Western blot
0.2-1 ug/mlELISA Titer: 1:12500Positive Control: Human brain| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
