Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :KIF5CPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :KIF5CSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: KIF5CSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain| Product Name | Rabbit anti-KIF5C polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ44735, KIAA0531, KINN, MGC111478, NKHC, NKHC-2, NKHC2 |
| Immunogen | The immunogen for anti-KIF5C antibody: synthetic peptide directed towards the N terminal of human KIF5C |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIF5C |
|---|---|
| Gene Synonyms | FLJ44735 KIAA0531 KINN MGC111478 NKHC NKHC-2 NKHC2 |
| Gene Full Name | Kinesin family member 5C |
| Gene Summary | KIF5C belongs to the kinesin-like protein family, Kinesin subfamily. It contains 1 kinesin-motor domain. Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. |
| Alternative Names | FLJ44735 KIAA0531 KINN MGC111478 NKHC NKHC-2 NKHC2 |
| Molecular Weight(MW) | 109kDa |
| Sequence | 957 amino acids |
Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :KIF5CPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :KIF5CSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: KIF5CSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
