Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: THP-1 cell lysate| Product Name | Rabbit anti-ETF1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | D5S1995, ERF, ERF1, MGC111066, RF1, SUP45L1, TB3-1 |
| Immunogen | The immunogen for anti-ETF1 antibody: synthetic peptide directed towards the N terminal of human ETF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanMouseZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ETF1 |
|---|---|
| Gene Synonyms | D5S1995 ERF ERF1 MGC111066 RF1 SUP45L1 TB3-1 |
| Gene Full Name | Eukaryotic translation termination factor 1 |
| Gene Summary | Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]). |
| Alternative Names | D5S1995 ERF ERF1 MGC111066 RF1 SUP45L1 TB3-1 |
| Molecular Weight(MW) | 49kDa |
| Sequence | 437 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: THP-1 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
