Immunoblot
Lane1: hMLH1 tranfected 293 cellsPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: MLH1Submitted by: Galina Petukhova, USUHSWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta| Product Name | Rabbit anti-MLH1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1 |
| Immunogen | The immunogen for anti-MLH1 antibody: synthetic peptide directed towards the N terminal of human MLH1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MLH1 |
|---|---|
| Gene Synonyms | COCA2 FCC2 HNPCC HNPCC2 MGC5172 hMLH1 |
| Gene Full Name | MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) |
| Gene Summary | This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC.This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Alternatively spliced transcript variants encoding different isoforms have been described, but their full-length natures have not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | COCA2 FCC2 HNPCC HNPCC2 MGC5172 hMLH1 |
| Molecular Weight(MW) | 84kDa |
| Sequence | 756 amino acids |
Immunoblot
Lane1: hMLH1 tranfected 293 cellsPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: MLH1Submitted by: Galina Petukhova, USUHS
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
