Western blot
Sample Type: Equine Cartilage Explants Primary Dilution: 1:1000 Secondary Antibody: Bio-Rad 170-5046 Secondary Dilution: 1:100,000Image Submitted By: Adam WilliamsUniversity of NottinghamWestern blot
Sample Type: 1. Normal HUman Cartilage (50ug)2. Human Osteoarthritis cartilage (50ug)3. Human Osteoarthritis chondrocytes (50ug)4. Human Osteoarthritis chondrocytes +IL-1 (50ug)Primary Dilution: 1:2000Secondary Antibody: HRP conjugated anti-rabbitSecondary Dilution: 1:5000Image Submitted By: Mukundan AtturNYU Hospital for Joint DiseaseWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Placenta| Product Name | Rabbit anti-MMP13 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLG3, MANDP1 |
| Immunogen | The immunogen for anti-MMP13 antibody: synthetic peptide directed towards the middle region of human MMP13 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMouseRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MMP13 |
|---|---|
| Gene Synonyms | CLG3 MANDP1 |
| Gene Full Name | Matrix metallopeptidase 13 (collagenase 3) |
| Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as art |
| Alternative Names | CLG3 MANDP1 |
| Molecular Weight(MW) | 42kDa |
| Sequence | 471 amino acids |
Western blot
Sample Type: Equine Cartilage Explants Primary Dilution: 1:1000 Secondary Antibody: Bio-Rad 170-5046 Secondary Dilution: 1:100,000Image Submitted By: Adam WilliamsUniversity of Nottingham
Western blot
Sample Type: 1. Normal HUman Cartilage (50ug)2. Human Osteoarthritis cartilage (50ug)3. Human Osteoarthritis chondrocytes (50ug)4. Human Osteoarthritis chondrocytes +IL-1 (50ug)Primary Dilution: 1:2000Secondary Antibody: HRP conjugated anti-rabbitSecondary Dilution: 1:5000Image Submitted By: Mukundan AtturNYU Hospital for Joint Disease
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Placenta| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
