Immunofluorescence
Sample type: zebrafish gut epithelial cellsGreen: primaryRed: actinBlue: DAPIPrimary dilution: 1:5000Secondary Dilution: 1:300Image Submitted by: Michel BagnatDuke University Medical Center See Customer Feedback tab for detailed information.Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Placenta| Product Name | Rabbit anti-RAB5B polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-RAB5B antibody: synthetic peptide directed towards the N terminal of human RAB5B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAB5B |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | RAB5B, member RAS oncogene family |
| Gene Summary | RAB5B play a role in protein transport. RAB5B is probably involved in vesicular traffic. |
| Molecular Weight(MW) | 24kDa |
| Sequence | 215 amino acids |
Immunofluorescence
Sample type: zebrafish gut epithelial cellsGreen: primaryRed: actinBlue: DAPIPrimary dilution: 1:5000Secondary Dilution: 1:300Image Submitted by: Michel BagnatDuke University Medical Center See Customer Feedback tab for detailed information.
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Placenta| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
