Immunofluorescence
Sample type: zebra gut epithelial cellsGreen: primaryRed: actinBlue: DAPIPrimary dilution: 1:5000Secondary Dilution: 1:300Image Submitted by: Michel BagnatDuke University Medical Center See Customer Feedback tab for detailed information.Immunofluorescence
Sample type: zebra gut epithelial cellsGreen: primaryRed: actinBlue: DAPIPrimary dilution: 1:5000Secondary Dilution: 1:300Image Submitted by: Michel BagnatDuke University Medical Center See Customer Feedback tab for detailed information.Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Muscle| Product Name | Rabbit anti-RAB5A polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAB5 |
| Immunogen | The immunogen for anti-RAB5A antibody: synthetic peptide directed towards the middle region of human RAB5A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAB5A |
|---|---|
| Gene Synonyms | RAB5 |
| Gene Full Name | RAB5A, member RAS oncogene family |
| Gene Summary | RAB5A is required for the fusion of plasma membranes and early endosomes. |
| Alternative Names | RAB5 |
| Molecular Weight(MW) | 24kDa |
| Sequence | 215 amino acids |
Immunofluorescence
Sample type: zebra gut epithelial cellsGreen: primaryRed: actinBlue: DAPIPrimary dilution: 1:5000Secondary Dilution: 1:300Image Submitted by: Michel BagnatDuke University Medical Center See Customer Feedback tab for detailed information.
Immunofluorescence
Sample type: zebra gut epithelial cellsGreen: primaryRed: actinBlue: DAPIPrimary dilution: 1:5000Secondary Dilution: 1:300Image Submitted by: Michel BagnatDuke University Medical Center See Customer Feedback tab for detailed information.
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Muscle| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
