Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Live| Product Name | Rabbit anti-PRKAR1A polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAR, CNC, CNC1, DKFZp779L0468, MGC17251, PKR1, PPNAD1, PRKAR1, TSE1, ADOHR |
| Immunogen | The immunogen for anti-PRKAR1A antibody: synthetic peptide directed towards the C terminal of human PRKAR1A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHumanMousePigRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKAR1A |
|---|---|
| Gene Synonyms | CAR CNC CNC1 DKFZp779L0468 MGC17251 PKR1 PPNAD1 PRKAR1 TSE1 ADOHR |
| Gene Full Name | Protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) |
| Gene Summary | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed. |
| Alternative Names | CAR CNC CNC1 DKFZp779L0468 MGC17251 PKR1 PPNAD1 PRKAR1 TSE1 ADOHR |
| Molecular Weight(MW) | 43kDa |
| Sequence | 381 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
