Western blot
Host: Rabbit Target Name: SIAH2 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: SIAH2 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/mlImmunoblot
1: 50ug HEK293T lysate, 2: 50ug A549 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:5000Gene Name: SIAH2Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, JenaWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Hela cell lysate| Product Name | Rabbit anti-SIAH2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hSiah2 |
| Immunogen | The immunogen for anti-SIAH2 antibody: synthetic peptide directed towards the N terminal of human SIAH2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SIAH2 |
|---|---|
| Gene Synonyms | hSiah2 |
| Gene Full Name | Siah E3 ubiquitin protein ligase 2 |
| Gene Summary | SIAH2 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | hSiah2 |
| Molecular Weight(MW) | 34kDa |
| Sequence | 324 amino acids |
Western blot
Host: Rabbit Target Name: SIAH2 Sample Tissue: MCF7 Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: SIAH2 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/ml
Immunoblot
1: 50ug HEK293T lysate, 2: 50ug A549 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:5000Gene Name: SIAH2Submitted by: Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Hela cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
