Immunohistochemistry
Immunohistochemistry with Tonsil tissue at an antibody concentration of 5ug/ml using anti-H2AFX antibodyWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: NCI-H226 cell lysate| Product Name | Rabbit anti-H2AFX polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H2A.X, H2A/X, H2AX |
| Immunogen | The immunogen for anti-H2AFX antibody: synthetic peptide directed towards the middle region of human H2AFX |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AFX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AX |
| Gene Full Name | H2A histone family, member X |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA |
| Alternative Names | H2A.X H2A/X H2AX |
| Molecular Weight(MW) | 16kDa |
| Sequence | 143 amino acids |
Immunohistochemistry
Immunohistochemistry with Tonsil tissue at an antibody concentration of 5ug/ml using anti-H2AFX antibody
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: NCI-H226 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM117569