Immunohistochemical
ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution :1:100Color/Signal Descriptions :Green: GFP Red: ACTN1Gene Name :ACTN1Submitted by :Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710Immunoblot
Lane1: 10 ug ACTN1-GFP transfected COS-7 lysateLane2: 10 ug ACTN2-GFP transfected COS-7 lysateLane3: 10 ug ACTN3-GFP transfected COS-7 lysateLane4: 10 ug ACTN4-GFP transfected COS-7 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: ACTN1Submitted by: Johannes W. Hell, University of CaliforniaWestern blot
ACTN1 antibody - N-terminal region validated by WB using 1.-2.Rat cortical neurons ctr siRNA (30ug)2. Rat cortical neurons ACTN1 siRNA (30ug) at 1:1000-1:2000.Western blot
Sample Type: 1.-4. rACTN1-GFP + HEK293 (50ug)Primary Dilution: 1:1000-1:2000Secondary Antibody: HRP conjugated goat anti-rabbitSecondary Dilution: 1:20,000Image Submitted By: Magdalena KalinowskaAlbert Einstein College of Medicine Â| Product Name | Rabbit anti-ACTN1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ40884 |
| Immunogen | The immunogen for anti-ACTN1 antibody: synthetic peptide directed towards the N terminal of human ACTN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMouseRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTN1 |
|---|---|
| Gene Synonyms | FLJ40884 |
| Gene Full Name | Actinin, alpha 1 |
| Gene Summary | Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Alternative Names | FLJ40884 |
| Molecular Weight(MW) | 103kDa |
| Sequence | 892 amino acids |
Immunohistochemical
ACTNX-GFP transfected COS-7 cellsPrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-Alexa-Fluor 568Secondary Antibody Dilution :1:100Color/Signal Descriptions :Green: GFP Red: ACTN1Gene Name :ACTN1Submitted by :Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710
Immunoblot
Lane1: 10 ug ACTN1-GFP transfected COS-7 lysateLane2: 10 ug ACTN2-GFP transfected COS-7 lysateLane3: 10 ug ACTN3-GFP transfected COS-7 lysateLane4: 10 ug ACTN4-GFP transfected COS-7 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: ACTN1Submitted by: Johannes W. Hell, University of California
Western blot
ACTN1 antibody - N-terminal region validated by WB using 1.-2.Rat cortical neurons ctr siRNA (30ug)2. Rat cortical neurons ACTN1 siRNA (30ug) at 1:1000-1:2000.
Western blot
Sample Type: 1.-4. rACTN1-GFP + HEK293 (50ug)Primary Dilution: 1:1000-1:2000Secondary Antibody: HRP conjugated goat anti-rabbitSecondary Dilution: 1:20,000Image Submitted By: Magdalena KalinowskaAlbert Einstein College of Medicine Â| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
