Immunohistochemistry
Immunohistochemistry with Rat Brain lysate tissueImmunohistochemistry
Immunohistochemistry with Heart cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-GPX4 antibodyWestern blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/mlWestern blot
GPX4 antibody - middle region validated by WB using HepG2 cell lysate at 1.0ug/ml.| Product Name | Rabbit anti-GPX4 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCSP, PHGPx, snGPx, snPHGPx |
| Immunogen | The immunogen for anti-GPX4 antibody: synthetic peptide directed towards the middle region of human GPX4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGoatGuinea PigHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPX4 |
|---|---|
| Gene Synonyms | MCSP PHGPx snGPx snPHGPx |
| Gene Full Name | Glutathione peroxidase 4 |
| Gene Summary | Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. |
| Alternative Names | MCSP PHGPx snGPx snPHGPx |
| Molecular Weight(MW) | 22kDa |
| Sequence | 197 amino acids |
Immunohistochemistry
Immunohistochemistry with Rat Brain lysate tissue
Immunohistochemistry
Immunohistochemistry with Heart cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-GPX4 antibody
Western blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/ml
Western blot
GPX4 antibody - middle region validated by WB using HepG2 cell lysate at 1.0ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
