Western blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Muscle Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: HIRIP3 Sample Tissue: 293T Antibody Dilution: 1.0ug/mlImmunoblot
1. 40 ug HK2 cell (kidney proximal tubular cell line) 2. 40 ug H2O2 treated human HK2 Cell lysate3. 40 ug H2O2 treated human HK2 Cell lysate4. 40 ug H2O2 treated human HK2 Cell lysate5. 40 ug H2O2 treated human HK2 Cell lysate6. 40 ug H2O2 treated human HK2 Cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: SOD2Submitted by: The University of Queensland, School of Medicine, Centre for Kidney Disease ResearchImmunoblot
1. 40ug rat dorsal medulla brain extract 2. 20 ug rat cortax + hypothalamus mitochondria extractPrimary Antibody Dilution: 1:2500Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: SOD2Submitted by: Manisha Nautiyal, Hypertension and Vascular Research Center, Wake Forest University School of MedicineWestern blot
Titration: 0.4 ug/mlPositive Control: Rat dorsal medulla brain & cortax + hypothalamus extractWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta| Product Name | Rabbit anti-SOD2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IPO-B, MNSOD, Mn-SOD, IPOB, MVCD6 |
| Immunogen | The immunogen for anti-SOD2 antibody: synthetic peptide directed towards the N terminal of human SOD2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRatSheepZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOD2 |
|---|---|
| Gene Synonyms | IPO-B MNSOD Mn-SOD IPOB MVCD6 |
| Gene Full Name | Superoxide dismutase 2, mitochondrial |
| Gene Summary | SOD2 is a member of the iron/manganese superoxide dismutase family. SOD2 is a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene encoding SOD2 have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Alternative Names | IPO-B MNSOD Mn-SOD IPOB MVCD6 |
| Molecular Weight(MW) | 24kDa |
| Sequence | 222 amino acids |
Western blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Brain Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: NOP56 Sample Tissue: Human Fetal Muscle Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: HIRIP3 Sample Tissue: 293T Antibody Dilution: 1.0ug/ml
Immunoblot
1. 40 ug HK2 cell (kidney proximal tubular cell line) 2. 40 ug H2O2 treated human HK2 Cell lysate3. 40 ug H2O2 treated human HK2 Cell lysate4. 40 ug H2O2 treated human HK2 Cell lysate5. 40 ug H2O2 treated human HK2 Cell lysate6. 40 ug H2O2 treated human HK2 Cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Goat anti-Rabbit HRPSecondary Antibody Dilution: 1:2000Gene Name: SOD2Submitted by: The University of Queensland, School of Medicine, Centre for Kidney Disease Research
Immunoblot
1. 40ug rat dorsal medulla brain extract 2. 20 ug rat cortax + hypothalamus mitochondria extractPrimary Antibody Dilution: 1:2500Secondary Antibody: Anti-Rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: SOD2Submitted by: Manisha Nautiyal, Hypertension and Vascular Research Center, Wake Forest University School of Medicine
Western blot
Titration: 0.4 ug/mlPositive Control: Rat dorsal medulla brain & cortax + hypothalamus extract
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
