Immunohistochemical
HeartWestern blot
Positive Control: Lane1: 25ug mouse NIH3T3 lysate, Lane2: 25ug mouse embryonic stem cell lysate, Lane3: 25ug mouse neural stem cell lysatePrimary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRSWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: OVCAR-3 cell lysate| Product Name | Rabbit anti-NES polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ21841, Nbla00170 |
| Immunogen | The immunogen for anti-NES antibody: synthetic peptide directed towards the middle region of human NES |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NES |
|---|---|
| Gene Synonyms | FLJ21841 Nbla00170 |
| Gene Full Name | Nestin |
| Gene Summary | Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neu |
| Alternative Names | FLJ21841 Nbla00170 |
| Molecular Weight(MW) | 177kDa |
| Sequence | 1621 amino acids |
Immunohistochemical
Heart
Western blot
Positive Control: Lane1: 25ug mouse NIH3T3 lysate, Lane2: 25ug mouse embryonic stem cell lysate, Lane3: 25ug mouse neural stem cell lysatePrimary Antibody Dilution : 1:500Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: OVCAR-3 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM117777