Western blot
Lanes :Lane 1: 20ug 143B lysate Primary Antibody Dilution : 1:1000 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution : 1:10,000 Gene Name :ATG4D Submitted by :Luis Anton, Universidad Autonoma de MadridWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HCT15 cell lysate| Product Name | Rabbit anti-ATG4D polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APG4-D, APG4D, AUTL4 |
| Immunogen | The immunogen for anti-ATG4D antibody: synthetic peptide directed towards the middle region of human ATG4D |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanPigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG4D |
|---|---|
| Gene Synonyms | APG4-D APG4D AUTL4 |
| Gene Full Name | ATG4 autophagy related 4 homolog D (S. cerevisiae) |
| Gene Summary | Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. |
| Alternative Names | APG4-D APG4D AUTL4 |
| Molecular Weight(MW) | 53kDa |
| Sequence | 474 amino acids |
Western blot
Lanes :Lane 1: 20ug 143B lysate Primary Antibody Dilution : 1:1000 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution : 1:10,000 Gene Name :ATG4D Submitted by :Luis Anton, Universidad Autonoma de Madrid
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HCT15 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
