Western blot
Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug U205 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Jose Luis Rosa, Universitat de BarcelonaWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain| Product Name | Rabbit anti-MAPK1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk |
| Immunogen | The immunogen for anti-MAPK1 antibody: synthetic peptide directed towards the middle region of human MAPK1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK1 |
|---|---|
| Gene Synonyms | ERK ERK2 ERT1 MAPK2 P42MAPK PRKM1 PRKM2 p38 p40 p41 p41mapk |
| Gene Full Name | Mitogen-activated protein kinase 1 |
| Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
| Alternative Names | ERK ERK2 ERT1 MAPK2 P42MAPK PRKM1 PRKM2 p38 p40 p41 p41mapk |
| Molecular Weight(MW) | 41kDa |
| Sequence | 360 amino acids |
Western blot
Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug U205 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Jose Luis Rosa, Universitat de Barcelona
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
