Western blot
Sample: SH-SY5Y cells 2.0x105 cells Primary dilution: 1:2500Secondary Antibody: HRP-conjugated goat anti-rabbit IgG Secondary dilution: 1:5000Image Submitted by: Atsuhiro TanabePharma Sci Kitasato UniversityWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: PANC1 cell lysate| Product Name | Rabbit anti-CASP6 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCH2 |
| Immunogen | The immunogen for anti-CASP6 antibody: synthetic peptide directed towards the middle region of human CASP6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP6 |
|---|---|
| Gene Synonyms | MCH2 |
| Gene Full Name | Caspase 6, apoptosis-related cysteine peptidase |
| Gene Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. |
| Alternative Names | MCH2 |
| Molecular Weight(MW) | 33kDa |
| Sequence | 293 amino acids |
Western blot
Sample: SH-SY5Y cells 2.0x105 cells Primary dilution: 1:2500Secondary Antibody: HRP-conjugated goat anti-rabbit IgG Secondary dilution: 1:5000Image Submitted by: Atsuhiro TanabePharma Sci Kitasato University
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: PANC1 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
