Immunoblot
Lane 1: Untreated human U251 cell lysateLane 2: IL-1beta treated human U251 cell lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: TLR4Submitted by: AnonymousWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: A549 cell lysate| Product Name | Rabbit anti-TLR4 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARMD10, CD284, TOLL, hToll |
| Immunogen | The immunogen for anti-TLR4 antibody: synthetic peptide directed towards the C terminal of human TLR4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR4 |
|---|---|
| Gene Synonyms | ARMD10 CD284 TOLL hToll |
| Gene Full Name | Toll-like receptor 4 |
| Gene Summary | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Also, several transcript variants of this gene have been found, but the protein coding potential of most of them is uncertain. |
| Alternative Names | ARMD10 CD284 TOLL hToll |
| Molecular Weight(MW) | 93kDa |
| Sequence | 839 amino acids |
Immunoblot
Lane 1: Untreated human U251 cell lysateLane 2: IL-1beta treated human U251 cell lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: TLR4Submitted by: Anonymous
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: A549 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
