Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HCT15 cell lysate| Product Name | Rabbit anti-USP8 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ34456, HumORF8, KIAA0055, MGC129718, UBPY |
| Immunogen | The immunogen for anti-USP8 antibody: synthetic peptide directed towards the N terminal of human USP8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | USP8 |
|---|---|
| Gene Synonyms | FLJ34456 HumORF8 KIAA0055 MGC129718 UBPY |
| Gene Full Name | Ubiquitin specific peptidase 8 |
| Gene Summary | USP8 is a hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. USP8 converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. USP8 catalytic activity is enhanced in the M phase. USP8 is involved in cell proliferation. USP8 is required to enter into S phase in response to serum stimulation. USP8 may regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. USP8 probably regulates the stability of STAM2 and RASGRF1. USP8 regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle.USP8 deubiquitinates EPS15 and controles tyrosine kinase stability.USP8 removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. USP8 is involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. |
| Alternative Names | FLJ34456 HumORF8 KIAA0055 MGC129718 UBPY |
| Molecular Weight(MW) | 127kDa |
| Sequence | 1118 amino acids |
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HCT15 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
