Western blot
Host: Rabbit Target Name: Uchl3 Sample Tissue: Mouse Testis lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-Uchl3 Antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for Anti-Uchl3 antibody is: synthetic peptide directed towards the middle region of Mouse Uchl3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Uchl3 |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| Gene Summary | Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Uchl3 has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Uchl3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Uchl3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance. Uchl3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome. |
| Molecular Weight(MW) | 26kDa |
| Sequence | 230 amino acids |
Western blot
Host: Rabbit Target Name: Uchl3 Sample Tissue: Mouse Testis lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
