Immunofluorescence
ImmunofluorescenceImmunoblot
Lane 1: 75,000 rat INS cellsPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: UCHL1Submitted by: Benedich Bracleva, VUB, Universitair Ziekenhuis BrusselWestern blot
Sample Type: 1. Rat brain cells (100ug)2. Rat Brain cells (100ug) incubatde with HA-UbVMEBand a: unmodified HA-UbVMEBand b: modified UCHL1Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal See Customer Feedback tab for detailed information.Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle| Product Name | Rabbit anti-UCHL1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARK5, PGP9.5, PGP95, Uch-L1, PGP 9.5 |
| Immunogen | The immunogen for anti-UCHL1 antibody: synthetic peptide directed towards the C terminal of human UCHL1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UCHL1 |
|---|---|
| Gene Synonyms | PARK5 PGP9.5 PGP95 Uch-L1 PGP 9.5 |
| Gene Full Name | Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| Gene Summary | The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. |
| Alternative Names | PARK5 PGP9.5 PGP95 Uch-L1 PGP 9.5 |
| Molecular Weight(MW) | 25kDa |
| Sequence | 223 amino acids |
Immunofluorescence
Immunofluorescence
Immunoblot
Lane 1: 75,000 rat INS cellsPrimary Antibody Dilution: 1:1000Secondary Antibody: Anti rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: UCHL1Submitted by: Benedich Bracleva, VUB, Universitair Ziekenhuis Brussel
Western blot
Sample Type: 1. Rat brain cells (100ug)2. Rat Brain cells (100ug) incubatde with HA-UbVMEBand a: unmodified HA-UbVMEBand b: modified UCHL1Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal See Customer Feedback tab for detailed information.
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
