Western blot
500 ug Human NT2 cell lysateAmount of IP Antibody :6 ugPrimary Antibody :VCPPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :VCPSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: VCPSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Live| Product Name | Rabbit anti-VCP polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IBMPFD, MGC131997, MGC148092, MGC8560, TERA, p97, ALS14 |
| Immunogen | The immunogen for anti-VCP antibody: synthetic peptide directed towards the C terminal of human VCP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHumanMousePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VCP |
|---|---|
| Gene Synonyms | IBMPFD MGC131997 MGC148092 MGC8560 TERA p97 ALS14 |
| Gene Full Name | Valosin containing protein |
| Gene Summary | The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. |
| Alternative Names | IBMPFD MGC131997 MGC148092 MGC8560 TERA p97 ALS14 |
| Molecular Weight(MW) | 89kDa |
| Sequence | 806 amino acids |
Western blot
500 ug Human NT2 cell lysateAmount of IP Antibody :6 ugPrimary Antibody :VCPPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :VCPSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: VCPSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Live| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
