Immunofluorescence
mouse 3T3 sWestern blot
Sample Type: 1. total HeLa cell extract (100ug)2. total HeLa cell extract incubated with HA-UbVME (100ug)3. Rat Liver cytosolic extract (100ug)4. Rat Liver cytosolic extract incubated with HA-UbVME (100ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, PortugalWestern blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 293T cell lysate| Product Name | Rabbit anti-USP9X polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DFFRX, FAF, FAM |
| Immunogen | The immunogen for anti-USP9X antibody: synthetic peptide directed towards the C terminal of human USP9X |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | USP9X |
|---|---|
| Gene Synonyms | DFFRX FAF FAM |
| Gene Full Name | Ubiquitin specific peptidase 9, X-linked |
| Gene Summary | This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Alternative Names | DFFRX FAF FAM |
| Molecular Weight(MW) | 292kDa |
| Sequence | 2570 amino acids |
| Entrez Gene | 8239 |
|---|---|
| SwissProt ID | Q93008 |
| Protein Accession | NP_001034679 |
Immunofluorescence
mouse 3T3 s
Western blot
Sample Type: 1. total HeLa cell extract (100ug)2. total HeLa cell extract incubated with HA-UbVME (100ug)3. Rat Liver cytosolic extract (100ug)4. Rat Liver cytosolic extract incubated with HA-UbVME (100ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 293T cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
