Immunofluorescence
Sample Type: complete mouse retina sectionsRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale UniversityWestern blot
1 ug/mlPositive Control: Fetal kidney lysate| Product Name | Rabbit anti-SYT5 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The immunogen for anti-SYT5 antibody: synthetic peptide directed towards the middle region of human SYT5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYT5 |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | Synaptotagmin V |
| Gene Summary | Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]). |
| Molecular Weight(MW) | 43kDa |
| Sequence | 386 amino acids |
Immunofluorescence
Sample Type: complete mouse retina sectionsRed: PrimaryBlue: DAPIPrimary Dilution: 1:200Secondary Antibody: Goat anti-Rabbit AF568 IgG(H+L)Secondary Dilution: 1:200Image Submitted by: David ZenisekYale University
Western blot
1 ug/mlPositive Control: Fetal kidney lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
