Immunohistochemistry
Application: IHCSpecies+tissue/cell type: Tadpole tegmenta horizontal sectionPrimary antibody dilution: 1:500Secondary antibody: Donkey anti-rabbit Alexa 488Secondary antibody dilution: 1:500Immunohistochemical
Species+tissue/cell type: Tadpole forebrain horizontal sectionPrimary antibody dilution: 1:500Secondary antibody: Donkey anti-rabbit Alexa 488Secondary antibody dilution: 1:500Immunohistochemical
CALB1 Antibody tested with human purkinje fibers in ImmunohistochemistryWestern blot
CALB1 antibody - C-terminal region validated by WB using COLO205 cells lysate at 1ug/ml.| Product Name | Rabbit anti-CALB1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CALB |
| Immunogen | The immunogen for anti-CALB1 antibody: synthetic peptide directed towards the C terminal of human CALB1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CALB1 |
|---|---|
| Gene Synonyms | CALB |
| Gene Full Name | Calbindin 1, 28kDa |
| Gene Summary | Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. |
| Alternative Names | CALB |
| Molecular Weight(MW) | 30kDa |
| Sequence | 261 amino acids |
Immunohistochemistry
Application: IHCSpecies+tissue/cell type: Tadpole tegmenta horizontal sectionPrimary antibody dilution: 1:500Secondary antibody: Donkey anti-rabbit Alexa 488Secondary antibody dilution: 1:500
Immunohistochemical
Species+tissue/cell type: Tadpole forebrain horizontal sectionPrimary antibody dilution: 1:500Secondary antibody: Donkey anti-rabbit Alexa 488Secondary antibody dilution: 1:500
Immunohistochemical
CALB1 Antibody tested with human purkinje fibers in Immunohistochemistry
Western blot
CALB1 antibody - C-terminal region validated by WB using COLO205 cells lysate at 1ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
