Western blot
Sample: SH-SY5Y cells 2.0x105 cellsPrimary dilution: 1:2500Secondary Antibody: HRP-conjugated goat anti-rabbit IgGSecondary dilution: 1:5000Image Submitted by:Atsuhiro TanabePharma Sci Kitasato UniversityWestern blot
MARCKS antibody - C-terminal region validated by WB using SHSY5Y cell lysate| Product Name | Rabbit anti-MARCKS polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL |
| Immunogen | The immunogen for anti-MARCKS antibody: synthetic peptide directed towards the C terminal of human MARCKS |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Pig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MARCKS |
|---|---|
| Gene Synonyms | 80K-L FLJ14368 FLJ90045 MACS PKCSL PRKCSL |
| Gene Full Name | Myristoylated alanine-rich protein kinase C substrate |
| Gene Summary | The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. |
| Alternative Names | 80K-L FLJ14368 FLJ90045 MACS PKCSL PRKCSL |
| Molecular Weight(MW) | 31kDa |
| Sequence | 332 amino acids |
Western blot
Sample: SH-SY5Y cells 2.0x105 cellsPrimary dilution: 1:2500Secondary Antibody: HRP-conjugated goat anti-rabbit IgGSecondary dilution: 1:5000Image Submitted by:Atsuhiro TanabePharma Sci Kitasato University
Western blot
MARCKS antibody - C-terminal region validated by WB using SHSY5Y cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
