Western blot
Positive Control: Lane 1: 50ug term baboon muscle homogenate Primary Antibody Dilution : 1:1666Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:1200Submitted by: Cynthia Blanco, University of Texas Health Science CenterWestern blot
Titration: 1.0 ug/mlPositive Control: A549 Whole Cell| Product Name | Rabbit anti-ACTA1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMouseRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTA1 |
|---|---|
| Gene Synonyms | ACTA ASMA CFTD CFTD1 CFTDM MPFD NEM1 NEM2 NEM3 |
| Gene Full Name | Actin, alpha 1, skeletal muscle |
| Gene Summary | The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. |
| Alternative Names | ACTA ASMA CFTD CFTD1 CFTDM MPFD NEM1 NEM2 NEM3 |
| Molecular Weight(MW) | 42kDa |
| Sequence | 377 amino acids |
Western blot
Positive Control: Lane 1: 50ug term baboon muscle homogenate Primary Antibody Dilution : 1:1666Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:1200Submitted by: Cynthia Blanco, University of Texas Health Science Center
Western blot
Titration: 1.0 ug/mlPositive Control: A549 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
