Immunoblot
Lane 1: 4ug DRM fraction from MDA-MB-231Primary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-rabbit IgG-HRPSecondary Antibody Dilution: 1:10000Gene Name: EGFRSubmitted by: Katarzyna Augoff, University of WroclawWestern blot
Titration: 1.0 ug/mlPositive Control: Placenta| Product Name | Rabbit anti-EGFR polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERBB, ERBB1, HER1, PIG61, mENA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGFR |
|---|---|
| Gene Synonyms | ERBB ERBB1 HER1 PIG61 mENA |
| Gene Full Name | Epidermal growth factor receptor |
| Gene Summary | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. |
| Alternative Names | ERBB ERBB1 HER1 PIG61 mENA |
| Molecular Weight(MW) | 69kDa |
| Sequence | 628 amino acids |
Immunoblot
Lane 1: 4ug DRM fraction from MDA-MB-231Primary Antibody Dilution: 1:1000Secondary Antibody: Donkey anti-rabbit IgG-HRPSecondary Antibody Dilution: 1:10000Gene Name: EGFRSubmitted by: Katarzyna Augoff, University of Wroclaw
Western blot
Titration: 1.0 ug/mlPositive Control: Placenta| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
