Western blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Product Name | Rabbit anti-VCL polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMD1W, CMH15, MVCL |
| Immunogen | The immunogen for anti-VCL antibody: synthetic peptide directed towards the C terminal of human VCL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanRabbitZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VCL |
|---|---|
| Gene Synonyms | CMD1W CMH15 MVCL |
| Gene Full Name | Vinculin |
| Gene Summary | Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. |
| Alternative Names | CMD1W CMH15 MVCL |
| Molecular Weight(MW) | 117kDa |
| Sequence | 1066 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
