Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Product Name | Rabbit anti-PPP1R12A polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MBS, MGC133042, MYPT1, M130 |
| Immunogen | The immunogen for anti-PPP1R12A antibody: synthetic peptide directed towards the C terminal of human PPP1R12A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseHumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPP1R12A |
|---|---|
| Gene Synonyms | MBS MGC133042 MYPT1 M130 |
| Gene Full Name | Protein phosphatase 1, regulatory subunit 12A |
| Gene Summary | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. |
| Alternative Names | MBS MGC133042 MYPT1 M130 |
| Molecular Weight(MW) | 113kDa |
| Sequence | 1030 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
