Immunohistochemistry
Immunohistochemistry with Small intestine tissue at an antibody concentration of 5ug/ml using anti-ROCK2 antibodyWestern blot
Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR BioscienceSecondry Antibody Dilution : 1:20,000Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
ROCK2 antibody - middle region validated by WB using OVCAR-3 cell lysate at 1.0ug/ml.| Product Name | Rabbit anti-ROCK2 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA0619, ROCK-II |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ROCK2 |
|---|---|
| Gene Synonyms | KIAA0619 ROCK-II |
| Gene Full Name | Rho-associated, coiled-coil containing protein kinase 2 |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. |
| Alternative Names | KIAA0619 ROCK-II |
| Molecular Weight(MW) | 153kDa |
| Sequence | 1388 amino acids |
Immunohistochemistry
Immunohistochemistry with Small intestine tissue at an antibody concentration of 5ug/ml using anti-ROCK2 antibody
Western blot
Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR BioscienceSecondry Antibody Dilution : 1:20,000Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
ROCK2 antibody - middle region validated by WB using OVCAR-3 cell lysate at 1.0ug/ml.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM118834