Immunoblot
1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: GCKSubmitted by: Received from annonymousWestern blot
Titration: 1.0 ug/mlPositive Control: COLO205 Whole Cell| Product Name | Rabbit anti-GCK polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2, LGLK |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GCK |
|---|---|
| Gene Synonyms | FGQTL3 GK GLK HHF3 HK4 HKIV HXKP MODY2 LGLK |
| Gene Full Name | Glucokinase (hexokinase 4) |
| Gene Summary | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). |
| Alternative Names | FGQTL3 GK GLK HHF3 HK4 HKIV HXKP MODY2 LGLK |
| Molecular Weight(MW) | 51kDa |
| Sequence | 464 amino acids |
Immunoblot
1: 50 ug HEP3B lysate, 2: 50 ug HEP3B lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: GCKSubmitted by: Received from annonymous
Western blot
Titration: 1.0 ug/mlPositive Control: COLO205 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
