Western blot
Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Jose Luis Rosa, Universitat de BarcelonaWestern blot
Titration: 1.0 ug/mlPositive Control: THP-1 Whole Cell| Product Name | Rabbit anti-PFKFB1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | F6PK, HL2K, MGC116715, MGC116717, PFRX |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PFKFB1 |
|---|---|
| Gene Synonyms | F6PK HL2K MGC116715 MGC116717 PFRX |
| Gene Full Name | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 |
| Gene Summary | This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. |
| Alternative Names | F6PK HL2K MGC116715 MGC116717 PFRX |
| Molecular Weight(MW) | 54kDa |
| Sequence | 471 amino acids |
Western blot
Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate Primary Antibody Dilution : 1:1000Secondary Antibody : Goat anti-rabbit-HRP Secondry Antibody Dilution : 1:5000Submitted by: Jose Luis Rosa, Universitat de Barcelona
Western blot
Titration: 1.0 ug/mlPositive Control: THP-1 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
